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Diaslide®: CLED/MacConkey #DS-101

 

DIASLIDE® C.L.E.D. / MacConkey Agar

Catalog No. DS-101

 

INTENDED USE

Diaslide® Urine Culture Device (UCD) is a semi-quantitative screening culture device for detecting, enumerating and identifying specific bacteria in urine. The device is intended for use in physician's office laboratories (POL) and clinical laboratories as an aid in the diagnosis of urinary tract infection (UTI).

 

SUMMARY AND EXPLANATION

Diaslide® UCD consists of a transparent, hinged plastic casing containing face-to-face plates of CLED and MacConkey agars with a plastic sampler having two bent tips located between.

               

CLED agar supports growth of both Gram-positive and Gram-negative bacteria, providing color changes that help in preliminary identification, especially of lactose-fermenting organisms, which change the color of the medium from green to yellow. The most significant lactose-fermenting bacteria involved in urinary tract infections are Escherichia coli, Klebsiella, Enterobacter and Enterococcus.   

 

CLED agar also supports Proteus and Pseudomonas, which do not ferment lactose. However, the low salt concentration of CLED agar inhibits the swarming of Proteus, which would otherwise obscure the observation of colonies.

 

MacConkey agar is a selective medium, giving an excellent differentiation between coliforms and non-lactose fermenters with inhibition of Gram-positive micrococci. Most urinary tract pathogens grow on it, whereas most contaminants are inhibited.

Current routine methods for bacteriological examination of urine are the classic Petri dish culture method and the and the dipslide technique. Diaslide® UCD combines the advantages of both techniques, enabling bacterial enumeration and isolation following a simple, user-friendly procedure.

 

PRINCIPLES OF THE PROCEDURE

The two bent tips of the sampler are dipped into the urine specimen and take up a standard volume of urine. The sampler is then pulled out through the casing, simultaneously inoculating the surfaces of both media with a streaking dilution. As a consequence, the number of bacteria deposited on the media is in direct proportion to the number of bacteria present in the specimen. Following incubation, the number of bacterial colonies cultures is compared with the Colony Density Chart to determine the level of bacteria in the urine sample.

 

KIT CONTENTS

Diaslide® UCD contains CLED and MacConkey agars.

 

CLED Medium

 

MacConkey Agar

 

Formula per liter

 

Formula per liter

 

Lab-Lenco Powder

3 g

Peptone

20 g

Peptone

4 g

Lactose

10 g

Tryptone

4 g

Bile salts No. 3

1.5 g

L-Cystine

0.128 g

Sodium Chloride

5 g

Lactose

10 g

Agar

15 g

Agar

15 g

Neutral Red

0.03 g

BromoThymol Blue

0.02 g

Crystal Violet

0.001 g

Final pH 7.3 ± 0.2 at 25°C

 

Final pH 7.2 ± 0.2 at 25°C

 

 

 

MATERIAL REQUIRED BUT NOT PROVIDED

Incubator (37 ± 1°C)

Incubation Stand

 

Click here to download full product insert

 

     FOR FURTHER INFORMATION EMAIL US OR CALL: +972-2-6781861

Product Documents
Click to Download : (226.1 Kb) Diaslide DS-101 Insert
Click to Download : (284.5 Kb) Diaslide Brochure

 
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