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UNI-SEP Lymphocyte Seperation Tubes

INTRODUCTION

Density gradient centrifugation of whole blood on a polysucrose - sodium metrizoate medium is the method of choice for isolation of lymphocytes. The success of the procedure, i.e. the recovery of viable lymphocytes with the lowest proportion of contaminating granulocytes and erythrocytes, depends to a large extent on the careful layering of the blood sample onto the separation medium and the maintenance of a sharp interface between the two solutions prior to centrifugation. 

To this end UNI-SEP was developed. The UNI-SEP system allows the blood sample to be poured directly into the centrifuge tube with no special precautions required to prevent disruption of the polysucrose - sodium metrizoate layer. Thus, a large number of samples may be handled at the same time. The mechanism also reduces the length of centrifugation time required for separation of the lymphocytes.

 

PRINCIPLE

UNI-SEP products are sterile plastic centrifuge tubes containing a solution of 5.6% polysucrose and 9.6% sodium metrizoate (density 1.077 g/ml, osmolality 280 mOsm), which is sequestered in the bottoms of the tubes by one-way plastic inserts. Blood poured onto the insert does not disrupt the surface of the polysucrose - sodium metrizoate layer. During centrifugation the insert opens to permit mixing of the blood and polysucrose - sodium metrizoate. Aggregated red blood cells and granulocytes are sedimented to the bottom of the tube while the white blood cells migrate to the interface between the plasma and polysucrose - sodium metrizoate layers. They are readily identified as a discrete white band located above the plastic insert.

 

FILLING OF TUBES:

 

Type of Tube

Cat. No.

Volume separation medium to be added

Uni-Sep

U-03

2 ml

Uni-Sep+

U-05

3 ml

Uni-SepMAXI

U-11

10 ml

Uni-SepMAXI+

U-17

15 ml

 

 

UNI-SEP: Comparison of major features with competing products

 

 

 

Leucoprep

LeucoSep

Accuspin

Uni-Sep

Manufacturer

Becton-Dickenson

Opopharma

Sigma

Novamed

Size

Two sizes, 15ml and 50ml tubes

Variable size, add separation medium as required

Same as Opopharma

Four different sizes, all with or without medium.

Separation medium

Tubes supplied with gel

Medium must be added before use

As Opopharma

Tubes supplied with or without medium, ready for use.

Separation technique

B-D's gel technology

Polyethylene porous disc

Polyethylene porous disc

Patented one-way valve, opening during centrifugation and closed otherwise.

Price

 

 

 

Significantly lower than all the others

 

 

  • It is a major time saver receiving the tubes with the separation medium already in place and the tubes ready for use.
  • Tubes supplied without this medium need to have the medium added (supplied separately and at added cost) followed by a centrifugation step before use to displace the medium under the porous barrier before use.
  • Our patented one-way barrier opens only under centrifugation and the monocyte fraction can be simply poured off after separation without fear of contamination by the trapped erythrocytes.

     FOR FURTHER INFORMATION EMAIL US OR CALL: +972-2-6781861


 

Product Documents
Click to Download : (38.8 Kb) UNI-SEP Insert
Click to Download : (329.5 Kb) UNI-SEP Comparison with competitors
Click to Download : (372.0 Kb) UNI-SEP Brochure
Click to Download : (49.7 Kb) UNI-SEP Unfilled Tubes Insert
Click to Download : (1.1 Mb) UNI-SEP Schematic Instructions

 
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