Buffy Booster for UNI-SEP # U-15
Buffy Booster for UNI-SEP
Catalog no. U-15
DENSITY GRADIENT LYMPHOCYTE SEPARATION FROM
BIOLOGICAL FLUIDS HAVING LOW RED CELL CONTENT
Critically important to the function of ready-to-use lymphocyte separation tubes (such as UNI-SEP) is the hematocrit, i.e. the volume of the blood fluid occupied by the red blood cells. It is this mass of cells that during the centrifugation procedure displaces the sodium metrizoate - polysucrose so that it rises above the centrifugation device to form a density layer at which the white cells collect. In cases where sufficient red cells are not present, the sodium metrizoate - polysucrose interface forms at or even below the level of the separation device. In this situation recovery of the white cell fraction may not be possible. Diluted whole blood, buffy coat (enriched white cell fraction), bone marrow, lymph and spinal fluid are all examples of this type of biological fluids.
When there is not sufficient red cell mass in the sample itself to displace the sodium metrizoate - polysucrose to the required level, extra mass must be added. Buffy Booster, a dense inert liquid, immiscible in water, is added prior to centrifugation. Centrifugal force causes Buffy Booster to sink to the bottom of the tube. Any red blood cells present form a sedimentation layer on top of the Buffy Booster (the red cell layer and Buffy Booster do not mix). The volume of sodium metrizoate - polysucrose displaced upwards is equal to the combined volumes of the Buffy Booster and the sedimented red cells. No contamination with red cells or Buffy Booster is possible even when the whole upper plasma layer is poured off. The red cells do not pass the separation device, while the Buffy Booster is blocked in by the red cell layer.
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