Diaslide®: URIselect 3/MacConkey #DS-102
DIASLIDE® URIselect 3 / MacConkey Agar
Catalog No. DS-102
Diaslide® Urine Culture Device (UCD) is a semi-quantitative screening culture device for detecting, enumerating and identifying specific bacteria in urine. The device is intended for use in physician's office laboratories (POL) and clinical laboratories as an aid in the diagnosis of urinary tract infection (UTI).
SUMMARY AND EXPLANATION
Diaslide® UCD (Columbia CNA/MacConkey) consists of a transparent, hinged plastic casing containing face-to-face agar plates. One plate contains Columbia CNA agar while the other contains MacConkey agar with a plastic sampler having two bent tips located between the two agar plates.
MACCONKEY AGAR is a selective and differential medium for detection of coliform organisms and enteric pathogens. The concentration of bile salts in this medium is relatively low in comparison with other enteric plating medium; therefore selectivity for gram-negative bacteria is not as great as in some other formulations. Crystal violet inhibits gram-positive microorganisms, especially enterococci and staphylococci. Differentiation of enteric microorganisms is achieved by the combination of lactose and the neutral red indicator. Colorless or pink to red colonies are produced depending upon the ability of the isolate to ferment lactose.
COLUMBIA CNA AGAR supplemented with 5% sheep blood is a selective and differential medium used for the isolation and differentiation of gram-positive microorganisms from clinical and non-clinical materials.
Current routine methods for bacteriological examination of urine are the classic Petri dish culture method
and the dipslide technique. Diaslide® UCD combines the advantages of both techniques, enabling
bacterial enumeration and isolation following a simple, user-friendly procedure.
PRINCIPLES OF THE PROCEDURE
The two bent tips of the sampler are dipped into the urine specimen and take up a standard volume of urine. The sampler is then pulled out through the casing, simultaneously inoculating the surfaces of both media with a streaking dilution. As a consequence, the number of bacteria deposited on the media is in direct proportion to the number of bacteria present in the specimen. Following incubation, the number of bacterial colonies cultures is compared with the Colony Density Chart to determine the level of bacteria in the urine sample.
Diaslide® UCD REF DS-102 units contain Columbia CNA agar and MacConkey agar.
MATERIALS REQUIRED BUT NOT PROVIDED
Incubator (37 ± 1°C) Incubation Stand
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